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Molecular characterisation of microsporidia causing nosematosis in bees
HANUŠOVÁ, Adéla
Microsporidia of the genera Nosema apis and Nosema ceranae cause bee intestinal disease, which is widespread throughout the world, including the Czech Republic. The aim of my bachelor thesis was to evaluate the occurrence of Nosema in various places in the Czech Republic. The literature contains only a limited amount of information about nosematosis caused by both microsporidia. In total, over 135 samples were taken from 27 sites. Samples were taken from November 2019 to January 2020. Sampling took place in the vicinity of large cities, in their vicinity, where the landscape was cultivated and in places with little agricultural impact. After analysis of the obtained samples by PCR, the vast majority of microsporidia of Nosema cerenae was present, while Nosema apis was nowhere.
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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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Infestace včelstev Nosema spp. v průběhu roku v různých lokalitách
DURČANSKÝ, Pavel
The nosem infection is a worldwide spread bee illness caused by two microsporidia (Nosema apis and Nosema ceranae). The aim of this thesis is to follow the occurrence of Nosema spp. and density of infection in the selected bee colonies considering the relation between N. apis, N. ceranae, climatic conditions and the condition of each bee colony concerned. In my thesis, I have used two ways of originator identification. The first was microscopy, using which we have discovered significant changes in the number of spores in one bee colony within one year. After the experiment had finished, we evaluated the number of measured spores in connection with the outside temperature, humidity, number of colonized frames, extenders, gentleness of bees and their sitting on the honey combs. The second way was using the PCR method. Through this method we have identified the percentage of positive bee colonies on the selected locations. Furthermore, we have confirmed the occurrence of each of the originators and evaluated if there is a mutual influence of Nosema spp. between bee colonies.
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The parasitic microorganisms in immunodeficient and healthy population of honebees (Apis mellifra)
Bičianová, Martina ; Hubert, Jan (advisor) ; Titěra, Dalibor (referee)
Immunodeficient honey bee (Apis mellifera) colonies suffer from broad range of parasites including eukaryotic protozoa. Despite this fact, the eukaryotic parasites are still poorly documented in the Czech Republic. The presence of eukaryotic parasites (Nosema ceranae, Nosema apis, Crithidia mellificae and Apicystis bombi) was observed in different apiaries in the Czech Republic. The samples were taken in 9 apiaries in 53 beehives during the 2014/2015 season. From each beehive, 10 adult of honey bees were taken from the peripheral comb in triplicate. DNA was isolated from every sample of honey bees. The parasites were detected by polymerase chain reaction (PCR) with specific primers. The treatment fall of parasitic mite Varroa destructor was obtained from beekeepers for season of 2014. Crithidia mellificae was detected by 5 types of specific primers (SEF, SER; SSU, SSU rRNA, Cyt b, Tryp cyt b) and positive amplicons were cloned and sequenced. The obtained sequences were compared with GeneBank and showed similarity from 98-100% to sequences of Lotmaria passim (Trypanosomatid). Crithidia mellificae was not detected. L. passim had prevalence of 79,2% and is reported in the Czech Republic for the first time. Primer Tryp-cyt b is recommended for the routine detection of L. passim. Nosema ceranae was...
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